IL-7Rα^hiCCR6⁺ lymphocytes are IL-17 producing cells.

<p>(A) CD44, CD62L and CXCR6 expression on digested lymph node cells from control or <i>Cxcr6^GFP/+</i> mice, gated on IL-7Rα^hiCCR6⁺ cells. (B) IL-17A staining of digested lymph node cells, stimulated with PMA/I for 2 h, gated on gated on IL-7Rα^hiCCR6⁺ cells. (C) αβT and γδT staining on digested lymph node cells, gated on IL-7Rα^hiCCR6⁺ cells; CD169 staining of αβT⁺, γδT⁺ and TCR⁻ IL-7Rα^hiCCR6⁺ cells gated as indicated in the left panel. (D) Flow cytometric detection of IL-7Rα^hiCCR6⁺ cells that bind CD1d-tetramers; far right panel shows CD169 staining on CD1d-tetramer⁺ IL-7Rα^hiCCR6⁺ cells. (E) CD169, CD4, and CD8 staining on digested lymph node cells, gated on IL-7Rα^hiCCR6⁺ cells. (F) Immunofluorescence microscopy of a lymph node from a wild-type or a CD4-deficient mouse, stained with anti-CD169 and anti-CD4 monoclonal antibodies. FO, follicle; T, T zone. Scale bar = 50 µm. All data are representative of at least two independent experiments.