Growth of the Δ<i>tcsC</i> mutant.

<p>Colonies of the AfS35 wild type and the Δ<i>tcsC</i> mutant grown for 72 h on AMM plates are shown in panels A/C and B/D, respectively. Magnifications of the edge of the colonies are depicted in panels C and D. Note the reduced number of extending hyphae in the mutant. Panel E: Quantification of the radial growth of AfS35 (black), Δ<i>tcsC</i> mutant (white) and complemented mutant colonies (gray) on AMM plates after 48 h and 96 h at 37°C or 48°C. Panel F: Quantification of the radial growth after 96 h of AfS35 (black), Δ<i>tcsC</i> mutant (white) and complemented mutant colonies (gray) on AMM plates supplemented with 1.4 M NaNO3 or 0.2 M ammonium tartrate at 37°C. The experiments shown in panels E and F were done in triplicate. Standard deviations are indicated. Student’s <i>t</i>-test: *p<0.005; **p<0.001.