tRNase Z^L is responsible at least partly for the reduction in the miR-16 level by sgR16(1–14).

<p>(A) Quantitation of the miR-16 level in HEK293 cells with a LightCycler 480 SYBR Green I Kit. The HEK293 cells were transfected with mock, 35 nM of sgR16(1–14) or sgR16(1–22) together with 50 nM of the scramble siRNA or the anti-tRNase-Z^L siRNA. sgR16(1–14) and sgR16(1–22) were phosphorylated at both 5′ and 3′ ends. The miR-16 levels are normalized against the 5S rRNA levels. Error bars indicate s.d. (n = 3). *, P<0.001. (B) Western blot analysis. The tRNase Z^L level under the above transfection conditions was analyzed. β-actin was used as a loading control.