Modulation of Bcl-xL expression is required for apoptotic effects of CLPTM1L.

<p>A) Western blotting for apoptotic regulators in A549 cells with CLPTM1L knockdown after treatment with cisplatin showing decreased Bcl-xL expression upon loss of CLPTM1L. Bcl-xL blots for two separate representative clonal populations of A549 cells with CLPTM1L knockdown (#1 and #2) are shown. B) Graphic representation of Bcl-xL expression in cells with CLPTM1L knockdown from three separate clonal populationsA549. Error bars represent one standard deviation from the mean. * - p<0.003 by Student’s T-Test C) Western blots confirming expression of exogenous Bcl-xL in A549 cells with CLPTM1L knockdown. D) Relative viable cell counts in A549 cells with CLPTM1L knockdown and ectopic Bcl-xL expression after treatment with cisplatin demonstrating the abolition of apoptotic effect of CLPTM1L loss upon ectopic Bcl-xL expression.