miR-21 regulates phosphorylation of tuberin and activation of mTOR in renal cancer cells.
- Nirmalya Dey (145124)
- Falguni Das (161719)
- Nandini Ghosh-Choudhury (47362)
- Chandi Charan Mandal (161724)
- Dipen J. Parekh (161729)
- Karen Block (161735)
- Balakuntalam S. Kasinath (145130)
- Hanna E. Abboud (161743)
- Goutam Ghosh Choudhury (145133)
Publication date
June 2012
DOIAbstract
<p>ACHN cells were transfected with miR-21 Sponge or vector. The cell lysates were immunoblotted with phospho-tuberin (Thr-1462), tuberin (panel A), phospho-S6 kinase (Thr-389), S6 kinase (panel B), phospho-mTOR (Ser-2448) and mTOR (panel C). miR-21 uses Rheb activation for TORC1 activity. ACHN cells were cotransfected with miR-21 Sponge and CA Rheb plasmid. The cell lysates were immunoblotted with phospho-S6 kinase (Thr-389), S6 kinase (panel D), phospho-mTOR (Ser-2448) and mTOR (panel E).</p
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