Bone Marrow Derived Cell Recruitment is Radiation Dose-Dependent.

<p>(<b>A,B</b>) Increasing levels of BMDC recruitment can be observed with increasing radiation doses on 2 photon laser microscopy <i>in-vivo</i> imaging 7 days post radiation (<i>Green: BMDC, blue: CD31-APC</i>), 10× magnification. (<b>A</b>) Single dose increase from 2Gy to 15Gy, (<b>B</b>) fractionated doses increase from 3*2Gy to 3*5Gy, all demonstrate an increase in BMDC recruitment. (<b>C</b>) Bar graph representing quantification of the extent of BMDCs recruited to site of cranial radiation 7 days post, demonstrating a statistically significant increase in BMDC recruitment between 2Gy to 6Gy and 2Gy to 15Gy, both p<0.0001***. Fractionated radiation demonstrates lower rate of BMDC recruitment when compared to its single fraction equivalent, perhaps explained by radiobiology principle of repair that occurs with fractionated radiation. The increase in BMDC recruitment is noted at all time points post radiation (1 hour, 1 day, 21 day).