Detection of intracellular cytokines/chemokines produced by CD8+ T cell memory subsets in response to stimulation with <i>S.</i> Typhi-infected autologous B-LCL.

<p>Histograms from a representative volunteer (53 s at day 7 post infection) showing the production of 6 cytokines/chemokines by memory T cell subsets following stimulation with <i>S</i>. Typhi-infected autologous B-LCL. CD69 (a marker of recent activation) is displayed on the y-axis and each of the 6 cytokines/chemokines measured are displayed on the x-axes. Column 1 represents total CD8+ T cells, column 2 represents T central memory (T_CM; CD62L+ CD45RA-), column 3 represents T naïve (T_N; CD62L+ CD45RA+), column 4 represents T effector memory (T_EM; CD62L- CD45RA-), and column 5 represents T effector memory CD45RA+ (T_EMRA; CD62L- CD45RA+). The percentage of positive cells is shown for the indicated region in each cytogram.