C57BL/6J mice elicited IL-6 associated inflammatory response to A/Mexico/4108/2009 (H1N1pdm) infection.

<p>Global gene expression profiling in lungs of C57BL/6J mice infected intranasally with 10⁵ EID₅₀ A/Mexico/4108/2009 (H1N1pdm). Probes for significantly differentially expressed genes were subjected to one-way hierarchical clustering analysis (Pearson’s correlation). The most significant gene networks (Inflammatory Response, Cell Growth and Metabolism) or most prominent represented canonical pathways (IL-1 and IL-6-mediated Inflammation, Interferon Response), as determined by IPA, are indicated for each cluster (A). Expression data of genes associated with IL-6 signalling, as determined by IPA, profiled over the course of infection (B_i). Visual representation of IL-6 signalling pathway, as represented by IPA, at day 3 pi. Overlaid colors represent gene regulation status; upregulated genes are red, downregulated genes are blue. Color intensity correlates with the magnitude of change in gene expression (B_ii). Gene expression data validation by qRT-PCR for <i>Il6</i>, IL-6 signalling genes <i>Stat3</i> and <i>Il6ra</i>, and IL-6 response genes <i>Orm2</i>, <i>Saa3</i>, <i>Saa4</i>, <i>Socs1</i>, and <i>Socs3</i>. Results are expressed as fold changes over expression in non-infected controls. Reported values for each time point are the average of three samples with +1 standard deviation indicated by vertical error bars. N.I. indicates non-infected controls. The two-tailed, two-sample unequal variances Student’s t-test was used to ascertain significance (p-value <0.05 = *, p-value <0.01 = **) (C).