CO-029 regulated cell-matrix and cell-cell adhesions.

<p>(<b>A</b>) Cell-matrix adhesion. Adhesion onto laminin 111, laminin 332, and fibronectin of HT29-NS and -KD cells was assayed after incubating at 37^oC in 5% CO₂ for 1 h. The adhesion of the KD cells on laminin 111 and laminin 332 was significantly increased compared with NS cells (<i>P</i> = .042 on laminin 111 and = .048 on laminin 332). (<b>B</b>) Total cell-cell adhesion. Cell aggregation was measured in the Ca⁺⁺-containing media after a relatively strong mechanical force was applied. (<b>C</b>) Ca⁺⁺-independent cell-cell adhesion. Cell aggregation was measured in the Ca⁺⁺-free media after relatively strong or mild mechanical forces were applied, respectively. The aggregation of NS and KD cells after shear stress were quantified as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0038464#s2" target="_blank">Materials and Methods</a>. <i>P</i> values are 0.03 for the aggregated cells in the presence of Ca⁺⁺, 0.001 for the area of aggregates in the presence of Ca⁺⁺, and 0.0004 for mild stress in the absence of Ca⁺⁺. Images of cell aggregates after the shear-stress treatment were obtained under phase-contrast microscopy. All of the data are projected as mean±SEM (n = 4). *<i>P</i><.05, **<i>P</i><.01.