Over-expression of CD82 inhibited invasion and migration of HTR8/SVneo cells.

<p>(<b>A, B</b>) Representative images of filters containing invaded cells in Matrigel invasion assay and transwell cell migration assay are shown. The statistical bar graphs show the summary of three independent experiments. (<i>t</i>-test) (<b>C</b>) Confirmation of over-expression of CD82 was shown by RT-PCR and Western blotting. GAPDH was used as an internal control in RT-PCR and a loading control in Western blotting. (<b>D</b>) MTT assay showed no significant difference on proliferation. (<i>t</i>-test) (<b>E</b>) Overexpression CD82 had no significant effect on apoptosis of HTR8/SVneo cells. (<i>t</i>-test) Mock: pFLAG-CMV4 empty vector, CD82: pFLAG-CMV4-CD82 vector.