Differential effect of 0.1 µM Cytochalasin-D treatment on membrane and filopodial adhesion.

<p>A) Time-lapse images from a real-time movie (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0038613#pone.0038613.s009" target="_blank">Movie S7</a>) showing a cell (initially at the trap focus, X) approaching a neurosphere. As the trap is moved away (panel IV), the cell is seen to remain adhered to the neurosphere. B) Time-lapse images from a real-time movie (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0038613#pone.0038613.s010" target="_blank">Movie S8</a>) of a single cell approaching a filopodium on the neurosphere. Despite close proximity (for up to 7 seconds), no filopodial adhesion takes place: the cell remains within the trap focal volume, X, as it is moved away from the neurosphere. The cartoon below panel B is a chimera depicting both phenomena (as cells numbered 1 and 2, respectively). The scale bar denotes 10 µm.