Steady-state ATPase kinetics for RecG.

<p>All measurements were carried out at 20°C in the presence 10 µM MDCC-PBP, 10 nM RecG and 500 nM DNA junction (A40:B40) in a buffer described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0038270#s4" target="_blank">materials and methods</a>. (a) <i>k</i>_cat and <i>K</i>_m for nucleoside triphosphates (b) <i>K</i>_i for non-hydrolyzable or slowly hydrolyzing nucleotides, using 10 µM ATP and varying inhibitor concentration.