<i>P. gingivalis</i>-induced proteolysis of RIPK proteins is dose-dependent and heat labile.

<p>HAEC were treated with medium (<b>M</b>), live <i>P. gingivalis</i> strain 381 (MOI 10) (<b>10</b>), live <i>P. gingivalis</i> (MOI 100) (<b>100</b>), heat-killed (HK) (60°C, 60 min) <i>P. gingivalis</i> 381 (MOI 100 equivalency) (<b>60°</b>), or with HK (80°C, 20 min) <i>P. gingivalis</i> 381 (MOI 100 equivalency) (<b>80°</b>) for 2 h. Whole cell lysates were analyzed for the detection of RIPK1 (left panel) or RIPK2 (right panel). Full-length RIPK1 and RIPK2 are indicated with arrows. Prominent <i>P. gingivalis</i>-induced LMW bands are indicated with asterisk(s). MW ladder is indicated on the left in kDa. GAPDH was detected as a loading control.