Histone H3K9/K14 acetylation pattern associated with the IFN-A gene promoters during virus infection.

<p>(<b>A</b>) Acetylated histone H3K9, acetylated H3K14 and histone H3 associated with the promoter and the coding region of the IFN-A2 gene were determined by quantitative ChIP assays in Namalwa B cells infected by Sendai virus. Mean and standard deviation values of enrichment fold was calculated as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0038336#s2" target="_blank">Materials and Methods</a>, with <i>p</i> values of statistical significance lesser than 0.01 indicated by **. These values derived from two replicate samples in two representative experiments. Anti-acetylated histone H3K9 and anti-acetylated H3K14 antibodies used in these experiments have shown to be highly specific for these acetylation sites <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0038336#pone.0038336-Suka1" target="_blank">[47]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0038336#pone.0038336-Fuchs1" target="_blank">[48]</a>. Anti-rabbit IgG serum was used as control of ChIP experiments. (<b>B</b>) Enrichment folds for the recruitment of GCN5 and PCAF to the IFN-A2 promoter were determined as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0038336#pone-0038336-g002" target="_blank">Figure 2</a>. Anti-goat IgG serum was used as control of ChIP experiments.