General caspase inhibitors z-VAD-FMK and Boc-D-FMK alter <i>P. gingivalis</i>-induced modification of RIPK1 and RIPK2 in HUVEC.

<p>HUVEC were pretreated (Pre-Tx) with medium (<b>M</b>), 0.25% DMSO vehicle control (<b>C</b>), 25 µM z-VAD-FMK (<b>VAD</b>), or 100 µM Boc-D-FMK (<b>Boc</b>) with for 1.5 h. HUVEC were then treated with medium (<b>M</b>) or <i>P. gingivalis</i> strain 381 (MOI 100, <b>381</b>) for 2 h. Whole cell lysates were analyzed for (<b>A</b>) RIPK1 or (<b>B</b>) RIPK2 and GAPDH. Full-length RIPK1 and RIPK2 are indicated with arrows. Prominent <i>P. gingivalis</i>-induced LMW bands are indicated with asterisks. MW ladder is indicated on the left in kDa.