Recruitment of HDAC3 to IFN-A gene promoters during virus infection.

<p>(<b>A</b>) Relative expression levels of HDAC3 were determined in Sendai virus-infected Namalwa cells by RT-QPCR as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0038336#pone-0038336-g001" target="_blank">Figure 1</a>. HDAC3 protein levels were analyzed in whole cell extracts prepared from Sendai virus-infected Namalwa B cells by Western blotting using HDAC3 and actin antisera. (<b>B</b>) Recruitment of HDAC3 and HDAC1 to the IFN-A2, A14 and A1 gene promoters was determined in Namalwa B cells infected by Sendai virus for 4 to 16 h by quantitative ChIP assays as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0038336#pone-0038336-g002" target="_blank">Figure 2</a>. Anti-rabbit IgG was used to determine non-specific binding.