Influences of expression of various PrP constructs on the cell viability and the relevant cellular factors.

<p>A. Cell viabilities assayed by CCK-8 kit. Each group is assigned to three repeating parallel units and measured with a spectrophotometer under 450 nm. The results are calculated from three independent tests and indicated as mean ± SD. Statistical differences compared with controls are illustrated as *P<0.05. B. Western blots. Cell lysates were separated in 15% SDS-PAGE and the specific immunoblots for Grp78, pro-caspase12, PDI, pro-caspase3, Bcl-2 and Bax were detected with individual antibodies. C. Quantitative analysis of each gray numerical value of Grp78, pro-caspase12, PDI, pro-caspase3 vs that of individual β-actin. The results are calculated from three independent tests and presented as mean ± SD. Statistical differences compared with controls are illustrated as *P<0.05. D. The relative densities of Bcl-2 and Bax specific bands normalized to β-actin are presented as ratio of Bax/Bcl-2. Statistical differences compared with controls are illustrated.