ROCK involvement in K8/K18 IF modulation of cell stiffness.

<p>(A) Mean bead displacement curves of one data set containing for 400 FU beads attached to a monolayer of cells seeded on a 3 kPa gel, following addition of Y27632 (1 µM, 30 min). (B) The corresponding cell elastic constant k_c obtained in presence of vehicle (Ctrl) or Y27632. The k_c difference between H4ev and shK8b cell treated with Y27632 is not statistically significant (p = 0.26) (C) Mean bead displacement curves of one data set containing for 125 FU beads attached to a monolayer of cells seeded in a FN-coated dish, following addition of Y27632. (D) The corresponding cell elastic constant k_c obtained in absence (Ctrl) or presence of Y27632. The k_c difference between H4ev and shK8b cell treated with Y27632 is not statistically significant (p = 0.40). The dotted lines correspond to the numerical fits on Y27632-treated cells, while the solid lines correspond to the numerical fit on control cells data. Bars denote SE. *, p<0.05 relative to controls.