IL-6 and IL-8 content of KLF2-transduced BOECs.

<p>(A) Immunofluorescence image showing co-localization of IL-6 (green) and VWF (red) in IL-1β-treated KLF2- and mock-transduced BOECs. Nuclei were visualized with DAPI (blue). Scale bars: 10 µm. (B) Western blot analysis for VWF, KLF2, IL-8 and IL-6 expression in lysates of mock- and KLF2-transduced BOECs; α-tubulin was shown as a loading control. (C) Immunofluorescence image showing co-localization of IL-8 (green) and VWF (red) in IL-1β-treated KLF2- and mock-transduced BOECs. Nuclei were visualized with DAPI (blue). Scale bars: 10 µm. (D) Release of VWF from PMA-stimulated KLF2 (black bars)- and mock (white bars)-transduced cells (IL-1β-treated), measured by determining the concentration of VWF in the conditioned medium by ELISA. **P<0.001; ***P<0.0001 by Students t-test (E-F) Release of IL-6 and IL-8 from PMA-stimulated KLF2 (black bars)- and mock (white bars)-transduced cells (IL-1β-treated), measured by determining the concentration of IL-6 and IL-8 in the conditioned medium by ELISA. The amount of IL-6 released without stimulation was slightly reduced in KLF2 expressing cells when compared to mock-transduced cells. NS: non-significant; *P<0.01; ***P<0.0001 by Students t-test.