Reduction of <i>Hox</i> gene expression in H1 TKO embryos.

<p>(A) Relative expression of <i>Hox</i> genes with altered mRNA levels in H1 TKO embryos compared with WT. Down-regulated <i>Hox</i> genes are located in <i>HoxA</i> (i), <i>HoxC</i> (ii), and <i>HoxD</i> (iii) clusters. Expression levels of <i>Hox</i> genes were analyzed by qRT-PCR and normalized over <i>GAPDH</i> and represented as a fold change between H1 TKO and WT embryos at E8.5. *: P<0.05, **: P<0.01, ***: P<0.001. Error bars: S.D. (B) The schematic representation of <i>Hox</i> gene clusters with expression patterns in H1 TKO embryos compared with WT.