The Gαq/11 proteins control both random and directed T cell migration.

<p>(<b>A</b>) Random migration on ICAM-1 of HSB2 T cells ± transfection with vector control or DN Gαq, DN Gα11 or both cDNAs showing a representative experiment of single cell tracking patterns (n = 15 cells per condition) and speed of migration (mean ±s.d.), n = 5 experiments; (<b>B</b>) Western blot showing expression level of Gαq ± treatment of HSB2 cells with either control siRNA or Gαq siRNA and α-tubulin to represent the sample loading control; (<b>C</b>) Primary T cells treated with either control siRNA or Gαq siRNA showing single cell tracking pattern (n = 15 cells per condition) and random migration on ICAM-1 (mean ± s.d.), n = 3 experiments; (<b>D</b>) Transwell assay showing extent of chemotactic response to CXCL12 of primary T cells treated with either control siRNA or Gαq siRNA, n = 3 experiments.