Rainbow trout clonal lines resistant and susceptible to <i>F. psychrophilum</i>.

<p>A) Comparative survival (%) of A3_r (resistant) and B57_s (susceptible) lines after infection with the JIP 02/86 strain of <i>F. psychrophilum</i>. Fish were challenged by intramuscular injection at different sizes and infectious doses. Survival was assessed when the mortality had stabilized (on day 32 for challenge 1 and on day 40 for challenge 2. Individuals used in the present study were the control sibs of challenge 2. B) Assessment of the bacterial load in the pronephros from the infected resistant and susceptible fish that were used for QPCR presented on <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0039126#pone-0039126-g004" target="_blank">figure 4</a>. The 16 S RNA was quantified using a real time QPCR validated for diagnostic of <i>F. psychrophilum</i>. Consistent results were observed from the fish used for micro array hybridization when tested (B57_s, n = 4, bacterial load 97 to 3372; A3_r, n = 3, bacterial load 0.003 to 6).