Histopathology and CD4⁺CD8⁺ thymocytes in the thymus.

<p>Mice (n = 6/group) were infected i.n. with 10⁴ PFU of V_K627, rV_K627E, Ts_E627, rTs_E627K, and PBS (mock mice). At 5 day p.i. the thymuses from each group were removed. (A) Three thymuses per group were processed for hematoxylin and eosin. Five sections per tissue were analyzed. Magnification, ×50. (B) Another three thymuses per group were prepared for single cell suspension. Following staining, the percentages of CD4⁺CD8⁺ thymocytes were examined by flow cytometry. Three independent experiments yielded consistent results.