NSL-bound genes display a specific nucleosome organization at their TSS.

<p>(A) The TSSs of constitutively active genes, either NSL-bound or –non-bound, were analyzed regarding their reported transcription initiation patterns <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002736#pgen.1002736-Ni1" target="_blank">[35]</a>. NSL-bound TSSs mostly belong to genes with weak and broad transcription initiation peaks (40% and 11.4%) whereas NSL-non-bound TSSs mainly belong to genes with narrow transcription initiation peaks (19.3%). (B) Boxplots of the sum of overlapping nucleosome reads in the regions 200 bp upstream and 200 bp downstream of the TSSs of constitutively expressed NSL-bound genes (red), constitutively expressed NSL-non-bound genes (gray), and tissue-specific genes (white). Genes were stratified based on their gene expression quartile (see <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002736#s3" target="_blank">Materials and Methods</a>) which demonstrates that the depletion of nucleosomes immediately upstream of the TSS that we observed for NSL-bound housekeeping genes (left side) is independent of expression levels (p-values for −200 bp region <2.2e-16; Wilcoxon test).