TRAIL induces activation of MAP Kinases is dependent on TRAF6.

<p>RAW264.7 cells were treated with TRAIL (500 ng/ml), M-CSF (20 ng/ml) and RANKL (50 ng/ml) in the presence or absence of TRAF6 siRNA. After stimulation, cells were solubilized, and cell lysates were subjected to Western blot analysis of TRAF6, p38 MAPK, JNK, and ERK1/2. The trace shown in the top panel for each group indicates the immunoreactivity of the phosphorylated kinase. The same membrane was then stripped and reprobed with the kinase antibody recognizing the total protein level of kinase (bottom panel). The results are representative of five separate experiments.