RPW-24 is toxic to <i>C. elegans</i>.

<p>(A) Wild-type <i>C. elegans</i> leave a lawn of <i>E. coli</i> containing 70 µM RPW-24 more so than DMSO after 16 hours of exposure. (B) The average percentage of wild-type <i>C. elegans</i> that were off a lawn of <i>E. coli</i> supplemented with either DMSO or 70 µM RPW-24 after 16 hours of exposure from three plates per condition [as in (A)] is presented with error bars representing SEM. <i>P</i> = 0.002 for the comparison of the two conditions. (C) RPW-24 shortens the lifespan of wild-type <i>C. elegans</i> in a dose-dependent manner. Data at each time point are the average of three plates per strain, each with approximately 50 animals per plate (sample sizes are given in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002733#pgen.1002733.s007" target="_blank">Table S2</a>). (D) RPW-24 slows development of wild-type <i>C. elegans</i>. Developmental stage was accessed in 50 animals per treatment group and presented as the percentage of the population that was at the indicated development stage after 65 hours of incubation at 20°C.