NuMA expression assessed by immunofluorescence reveals that NuMA localises to the nucleus during interphase and to the spindle poles during mitosis in cells of ovarian cancer cell lines and primary ascites cultures and fluorescence intensities vary among the primary cultures.

<p>A. Examples of interphase nuclei with low (a-d) and high (e-h) NuMA levels, and mitotic spindles with low (i-l) and high (m-p) NuMA levels at spindle poles. Green – NuMA, red – α-tubulin, blue - DAPI. Scale bar = 5 µm. B. Histogram comparing NuMA nuclear fluorescence staining intensities in four ovarian cell lines, one cell line established from a primary culture (GYNA0089) and 23 primary cultures derived from ascitic fluid. C. Histogram comparing NuMA staining intensities at spindle poles in the subset of cell lines and cultures where a sufficient number of mitotic cells were for analysis.