Evaluation of tumor vasculature by PECAM-1 immunostaining.

<p>Nude mice were subcutaneously injected with AsPC-1 cells (0.75×10⁶) and treated with Gem, Bev, Su and EMAP, either alone or in combination, for 2 weeks. (A) Tumor tissue sections were stained with PECAM-1 antibody and photographed under a fluorescent microscope. (B) PECAM-1 positive microvessel were counted in five different high power fields. The data are expressed as the mean ± standard deviation. Symbols • and * represent significant differences (P<0.05) compared with controls and Gem group, respectively.