The NG50 of ccTSA on datasets from 4 organisms with different sequence coverage and k-mer values.

<p>(A) E.coli, Exact error model (Exact), and 36 bp reads (36 bp), (B) E.coli, Illumina error model (Illumina), and 36 bp, (C) E.coli, Exact, and 75 bp reads (75 bp), (D) E.coli Illumina, and 75 bp, (E) S.cerevisiae, Illumina, and 75 bp, (F) L.major, Illumina, and 75 bp, and (G) C.elegans, Illumina, and 75 bp. The k-mer values were varied from 19 to 35 on 36 bp data and from 37 to 73 on 76 bp data. In most datasets, NG50 values increased then decreased as we increased the k-mer values. The NG50 values were mostly saturated on the sequence coverage of 80x. The longer the genome size of an organism, the lower its NG50 values were.