TRPA1 is critical for sensory nerve detection of PUFAs.

<p>(A) Fluo-4 fluorescence images of DHA-responsive neurons (top panel) and a non-responsive neuron (bottom panel). Scale bar is 40 µm. (B&C) Representative Ca²⁺ transients evoked by DHA (100 µM), AITC (1 mM), KCl (140 mM) in DRG neurons (<i>B</i>, DHA responsive; <i>C</i>, DHA-unresponsive). (D) Ca²⁺ transients evoked by DHA (100 µM) or capsaicin (CAP, 100 nM) in DRG neurons from TRPA1-null mice. (E) The percentage of WT or TRPA1-null neurons responsive to DHA (WT, n = 25 of 143; TRPA1-null, n = 1 of 60), LA (WT, n = 2 of 58), AITC (WT, n = 39 of 201) or CAP (TRPA1-null, n = 16 of 60).