Morphology of HT1080 cells cultured on tissue culture plates coated with BSA, intact LM111 or rLCC111 (10 mg/ml).

<p>Cells were allowed to attach for 6 hours, followed by fixation and staining with Alexa Fluor 594 phalloidin (A). Scale bar is 10 µm. Spread and non-spread cells were counted and percentages of non-spread cells are indicated (B). Spread cells were defined as large cells with extensive visible lamellipodia, whereas non-spread cells were defined as round cells with little or no membrane protrusions. Spread and non-spread cells were counted in four high-power fields and represented as mean±SD for each condition and time point. The data were evaluated by <i>t</i> test and were considered to be statistically significant when <i>p</i>≤0.05. (*, <i>p</i><0.05; **, <i>p</i><0.005, ***, <i>p</i><0.005). Representative phase-contrast images are shown from one of three independent experiments (C).