KLF4-expressing hair follicle stem cells were examined by lineage tracing.

<p>KLF4/CreER™/Rosa26RLacZ mice were induced by tamoxifen (100mg/kg) for 5 consecutive days at 6-week-old. 4 weeks later X-gal staining was performed. Potential KLF4 expression in interfollicular epidermis (shown by red arrows in B, C) and bulge area (A, and black arrows in B, C) was shown. A typical epithelial proliferation unit was shown in E (inset). Control staining was shown in D using KLF4/CreER™/Rosa26RLacZ mice with mock induction. Representative images were shown from 5 mice in each treatment group. Note that fixation was performed without xylene in A and B. Scale bars, 80 µm.