Interpretation for the role of actin on WASH dynamics on and off endosomes.

<p>To explain how actin depolymerization increases the steady state amount of WASH on endosomes and decreases the exchange of endosomal WASH for cytosolic WASH, we propose that actin branched networks promote the detachment of WASH from endosomes. For example, endosomal WASH may bind to the active Arp2/3 complex at branched junctions of the actin network, and detach from the endosome as the branched junction moves backward because of filament elongation.