Electroretinographic responses from <i>Tpst1^−/−</i> and <i>Tpst2^−/−</i> retinas.

<p><b>A.</b> Representative waveforms recorded at different light intensities from <i>wt</i>, <i>Tpst1^−/−</i> or <i>Tpst2^−/−</i> retinas under scotopic conditions. <b>B & C,</b> in each panel, the dashed line represents the 95% confidence interval for responses obtained from 3 150-day-old <i>wt</i> littermate controls. The a- and b-waves obtained from 3 150-day-old <i>Tpst1^−/−</i> mice (squares) fell near the lower limit of this interval at lower light intensities, but were well within the range at higher light intensities. In contrast, a- and b-waves recorded from 3 123-day-old <i>Tpst2^−/−</i> mice (triangles) were reduced in amplitude and fell outside the 95% confidence interval at all light intensities tested. Development of the (<b>D</b>) scotopic a-wave, (<b>E</b>) scotopic b-wave, and (<b>F</b>) photopic b-wave responses for <i>wt</i> (dotted line), <i>Tpst1^−/−</i> (squares), and <i>Tpst2^−/−</i> (triangles) mice. Note that all responses from <i>Tpst2^−/−</i> mice were lower than those for <i>Tpst1^−/−</i> and <i>wt</i> mice at all ages. Error bars represent standard error of the mean. The differences between <i>wt</i> and <i>Tpst2^−/−</i> presented in <b>D</b> are statistically significant between P30 and P300 (P<0.05–0.001) while the differences between <i>wt</i> and <i>Tpst1^−/−</i> are only statistically significant at P30 and P60 (P<0.05–0.001). The differences between <i>wt</i> and <i>Tpst2^−/−</i> presented in <b>E</b> are statistically significant between P60 and P600 (P<0.01–0.001) while the differences between <i>wt</i> and <i>Tpst1^−/−</i> are only statistically significant at P60 (P<0.05). The differences between <i>wt</i> and <i>Tpst2^−/−</i> presented in <b>F</b> are statistically significant for all time points tested (P<0.05–0.001) while the differences between <i>wt</i> and <i>Tpst1^−/−</i> are statistically insignificant for all time points tested. Number of animals tested in <b>D, E & F</b> are presented in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0039702#pone-0039702-t003" target="_blank">Table 3</a>.