Quantitative analysis of the effect of the <i>pch2</i> mutation on DSB formation.

<p>(A, B) the relationship between DSB formation and chromosome size in the <i>sae2</i> (A) and <i>dmc1</i> (B) backgrounds. DSB formation was expressed by the percentage of broken chromosomes (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0039724#pone-0039724-g001" target="_blank">Figure 1C and F</a>, and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0039724#s4" target="_blank">Materials and Methods</a>). Welch two sample t-test was used to analyze the effect of the <i>pch2</i> mutation on DSB formation. The break rate on each chromosome was deduced from the procedure described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0039724#s4" target="_blank">Materials and Methods</a> and Supplemental material. The effect of the <i>pch2</i> mutation on DSB formation is significant in both the <i>sae2</i> background (t = 3.8661, p-value = 0.0005509) and the <i>dmc1</i> background (t = 2.5528, p-value = 0.01707). (B, D) DSB amount in the <i>PCH2</i> positive strain was divided by that of the <i>pch2</i> negative counterpart per each chromosome in either <i>sae2</i> (C) or <i>dmc1</i> (D). (E) Quantitative evaluation of the <i>pch2</i> effect on DSB formation. The number of DSB forming events was calculated as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0039724#s4" target="_blank">Materials and Methods</a> and Supplemental material. At least two experiments were done for each data point. Error bars represent standard error.