Effect of transfection with control-siRNA on the phosphorylation of IKKα and IKKβ.

<p>U87, human glioma cells were transiently transfected with control-siRNA. Cells were induced with 10 ng/ml TNF-α for 0,5,10,20,40,60 min and treated with DMA (50 µM) and/or irradiated at 8.5 Gy. Phosphorylation of IKKα, IKKβ was observed in DMA treated cells, alone or in combination with IR.