DMA attenuates radiation induced apoptosis in U87 through NIK.

<p>(A) Apoptosis analysis of control siRNA transfected U87 cells at 3 h, 6 h in different treatment condition i.e. 50 µM DMA, radiation (8.5 Gy) and DMA (50 µM) + Radiation (8.5 Gy). Percentage of apoptosis is defined by % of cells that are Annexin V⁺ and Annexin V ⁺PI⁺. (B) Apoptosis analysis of siRNA-NIK transfected U87 cells at 3 h, 6 h in different treatment condition i.e. 50 µM DMA, radiation (8.5 Gy) and DMA (50 µM) + Radiation (8.5 Gy). Percentage of apoptosis is defined by % of cells that are Annexin V⁺ and Annexin V ⁺PI⁺. (C) Percentage of Annexin V⁺ and Annexin V ⁺PI⁺.positive cells in control siRNA transfected U87 cells at, 3 h,6 h, and 24 h in different treatment condition i.e. 50 µM DMA, radiation (8.5 Gy) and DMA (50 µM) + Radiation(8.5 Gy). (Data Not shown for 6 h and 12 h for only DMA and only radiation treatment condition). (D) Percentage of ofAnnexin V⁺ and Annexin V ⁺PI⁺.positive cellssiRNA-NIK transfected U87 cells at 3 h, 6 h, and 24 h in different treatment condition i.e. 50 µM DMA, radiation (8.5 Gy) and DMA (50 µM) + Radiation (8.5 Gy).(Data Not shown for 6 h and 12 h for only DMA and only radiation treatment condition). (E) Time course graphs showing the progression of cell cycle of control siRNA transfected U87 cells at 0, 3, 6, 12, 18 and 24 h in different treatment condition i.e. 50 µM DMA, radiation (8.5 Gy) and DMA (50 µM) + Radiation(8.5 Gy). (F) Time course graphs showing the progression of cell cycle of siRNA-NIK transfected U87 cells at 0, 3, 6, 12, 18 and 24 h in different treatment condition i.e. 50 µM DMA, radiation (8.5 Gy) and DMA (50 µM) + Radiation(8.5 Gy). (G) Cell cycle progression of control siRNA transfected U87 cells showing % G2-M fraction at 0, 3, 6, 12, 18 and 24 h in different treatment condition i.e. 50 µM DMA, radiation (8.5 Gy) and DMA (50 µM) + Radiation(8.5 Gy). (H) Cell cycle progression of siRNA-NIK transfected U87 cells showing % G2-M fraction at 0, 3, 6, 12, 18 and 24 h in different treatment condition i.e. 50 µM DMA, radiation (8.5 Gy) and DMA (50 µM) + Radiation(8.5 Gy).