Luciferase assays for NFκB activation in U87 cells.

<p>Cells were co-transfected with pNFκB-Luc and pRenilla. After 24 h, cells were irradiated (8.5 Gy) with or without DMA treatment (50 µM) and stimulated with TNF-α (20 ng/ml) for 4 h prior to cell lysis. Luciferase activity was determined to quantify NFκB transcription activity. Relative Luciferase activity (RLA) for NFκB activation was increased in DMA treated cells compared with irradiated or control cells. The triplicate samples were used and the data was presented as mean±SD from three independent experiments. (*indicates statistical significance by T-test, *p<0.00001).