Tissue-specific analysis of the TcOPT3.

<p>Real-time RT-PCR expression analysis of the <i>TcOPT3</i> gene expression in roots (R), leaves (L) and stems (S). The ΔCp values were calculated as follows: CP of target gene (<i>TcOPT3</i>) – CP of constitutive control gene (<i>ubiquitin-conjugating enzyme</i>), where the CP value is the fractional cycle number of crossing point (CP). The ΔCP values represent the mean of three technical replicates (±SD) of one experiment representative of three independent experiments. Relative transcript levels (RTL) were calculated as follows: RTL = 2^−ΔCP.