Microfluidic cell confinement device.

<p>(a) Device schematic with inset of confinement assay. The posts are 20×80 µm spaced equally 40 µm apart. (b) In the unconfined state, the posts are raised from the glass substrate (top), upon applying pressure the confinement chamber is compressed such that the posts are in contact with the glass substrate. (c) Seeding cells in the confinement chamber (left), when the posts are lowered the cell is confined to 3 or 7 µm height and is squeezed out from the post area (middle). Cells spread and attach in the confined volume (right).