Centromere and telomere behaviour during prophase I.

<p>(A) Leptotene nucleus with 28 rendered telomere signals (red), 7 rendered centromere signals (pink) and diffuse, polarised ASY1 signals (green). (B) Leptotene nucleus with a cluster of 7 rendered telomere signals (red), 7 rendered centromere signals (pink) and linearising elements of ASY1 (green) in the same hemisphere as the telomeres. (C) FISH to an embedded leptotene nucleus with a Rabl orientation of 19 rendered telomere signals (red) and 5 rendered centromere signals (pink), and 2 separate single-locus BAC DH053N18 signals (green). (D) FISH to an embedded leptotene nucleus with a bouquet orientation of 7 rendered telomere signals (red) and 7 rendered centromere signals (pink), and 2 separate single-locus BAC DH053N18 signals (green). (E) FISH to a squashed pachytene nucleus showing centromeres (red) and a single BAC signal (green). (F) Enlargement of red box shown in E, showing the close proximity of the BAC (green) to the centromere (red). All images are deconvolved maximum projections of nuclei, and are counterstained with DAPI (blue). (A–B) were imaged by CLSM, and (C–G) by wide-field fluorescence microscopy. (A–D) For ease of counting, the positions of centromeric and telomeric FISH signals have been marked by rendered spears using Imaris, an un-rendered nucleus is shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0039539#pone.0039539.s001" target="_blank">Figure S1A</a>.