Analysis of synaptic progression during barley prophase I.

<p>(A & E) Deconvolved maximum projection of a zygotene nucleus embedded in polyacrylamide and captured using CLSM, showing ASY1 cores (green) and ZYP1 cores (red). (B) Zygotene nucleus shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0039539#pone-0039539-g002" target="_blank">Figure 2A</a> processed using Imaris with each of the seven synapsing bivalents isolated, showing generated surfaces for ASY1 (green) and ZYP1 (red). (C) A single bivalent extracted from the reconstruction in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0039539#pone-0039539-g002" target="_blank">Figure 2B</a>, containing an example of a ZYP1 focus present on a single AE (white box). (D) Detail of an interlock isolated from the nucleus shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0039539#pone-0039539-g002" target="_blank">Figure 2A</a>. For ease of interpretation, one of the bivalents has been re-coloured to show ASY1 in grey and ZYP1 in black. (E) Zygotene nucleus containing a telomere cluster delimited by darker staining DAPI (blue) and emanating Zyp1 cores identified by the white arrow. (F) Stacked bar graph showing the cumulative lengths of ZYP1 and ASY1 fragments constituting each bivalent isolated from 9 nuclei. Bivalents from the same nucleus are grouped together and ordered by descending length. Bivalent complements are ordered by ascending average percentage synapsis shown below the groups.