Inhibition of CLIC4 by siRNA enhanced autophagy under starvation conditions in U251 cells.

<p>U251 cells were transfected with a plasmid encoding empty vector or CLIC4 siRNA vector for 24 h. (A) LC3 immunostaining (Hoechst 33342 staining for the nucleus, 600×) and MDC staining (900×) were observed by confocal microscopy in U251 cells incubated with EBSS for 8 h. (B) Western blot analysis of LC3 expression in U251 cells incubated with EBSS for 8 h. (C) The ratio of LC3-II/β-actin. Data were presented as a mean ± SD of three independent experiments. ^*<i>P</i><0.05 versus control group and ^#<i>P</i><0.05 versus starvation group.