Morphological and anatomical characteristics of sheathed ericoid mycorrhiza synthesized <i>in vitro</i> and observed under natural conditions.

<p>Sheathed ericoid mycorrhiza was synthesized <i>in vitro</i> between the basidiomycetous mycobiont (strains JPK 87 and JPK 90) and European blueberry (<i>Vaccinium myrtillus</i>) seedlings or observed in European blueberry hair roots collected in field. 3A) Terminal part of hair root is covered by a dense hyphal sheath (arrow). JPK 87, DIC, stained with trypan blue, bar = 100 µm. 3B) Detail of a dense hyphal sheath (HS) covering terminal part of a hair root. Note the extensive extraradical hyphae (EH). JPK 90, DIC, stained with trypan blue, bar = 50 µm. 3C) Dense intracellular hyphal coils (arrows) developing in the rhizodermal cells below a hyphal sheath. JPK 90, DIC, stained with trypan blue, bar = 20 µm. 3D) Clamp connection (arrow) formed by the basidiomycete inside a rhizodermal cell. Roots from field, DIC, bar = 10 µm. 3E) Terminally swollen capitate cystidium emerging from a sheathed ericoid mycorrhiza. Roots from field, DIC, bar = 10 µm. 3F) Vigorous intracellular colonization (arrows) by the basidiomycete accompanied by numerous extraradical hyphae (EH). JPK 90, DIC, bar = 50 µm.