Loss of Tgfbr2 in neonatal endothelial cells leads to cerebral vascular pathologies and intracerebral haemorrhage.

<p>Coronal sections through cerebral cortices of P14 control (A) and Tgfbr2–iKO^e mice (B) were stained with H&E. Note the abnormal blood vessel morphologies and microhaemorrhage in Tgfbr2 mutant brains. (C-H); Coronal sections through cerebral cortices of P14 control (C, E, G) and Tgfbr2 conditional mutant mice (D, F, H) were double immunofluorescently labelled with anti-laminin (C, D) and anti-GFAP (E, F) to visualize vascular basement membranes and astrocytes, respectively. Note the cerebral blood vessels with glomeruloid-like tufts (D) as well as robust perivascular astrogliosis (F) in Tgfbr2-iKO^e mutant sections. A total of 5 mutant and 4 control mice were examined for brain pathologies. Scale bars: 50 µm.