10 µM SB-216763 maintains MilliTrace Nanog GFP Reporter mESCs in a pluripotent-like state for more than a month.

<p>(A) GFP fluorescence in representative colonies from each treatment. (B) Mean pluripotency rating of two independent experiments ± SEM. Forty colonies were rated from each condition for the intensity and uniformity of GFP fluorescence after a month in culture. Statistical analysis revealed that the cultures maintained with LIF, 3 µM and 10 µM SB-216763 were significantly different than mESCs maintained without LIF.