Induction of K15 transcription in keratinocytes cultured at increasing cell densities.

<p>N-Terts growing in SFM at low (0.09 mM) (a) and high (1.8 mM) (b) calcium concentrations were allowed to reach the desired confluence before being used for expression of K1, K10, K14, K15, cornifin and FOXM1B. In (c) N-Terts at low calcium (0.09 mM) were plated at the required density, allowed to attach for 24 h, after which they were used to measure mRNA levels by qPCR. Each bar represents the mean±SEM where n = 3.