shRNA effect on target expression.

<p>Kinase transcript levels in LNCaP (A) and C4-2B (B) cells following knockdown with two independent shRNAs per kinase. Transcript levels were measured by qPCR on day 4 following transduction and 2 hours after R1881 hormone treatment (vehicle, 0.05, 0.5, and 1 nM). Transcript levels were compared to untreated pLKO control and normalized to the housekeeping gene, PSMB6. Values were averaged across hormone concentrations and biological replicates. Error bars represent standard error of the mean. ∗ denotes statistical significance.