A type III strain expressing type I or type II ROP18 inhibits Irgb6 accumulation and killing.

<p>WT MEFs were stimulated for 24 hours with IFNγ and infected with type I (GT1), type II (Pru), type III (CEP), CEP + ROP18_I or CEP + ROP18_II expressing GFP. (A) Cells were fixed after 1 hour and stained by immunofluorescence for Irgb6 (Red) and with Hoechst (blue). Scale bar represents 5 µm. (B) Quantification of Irgb6 localization on the parasite containing vacuole and percentage plaque loss after 4–7 days on stimulated MEFs compared to unstimulated MEFs. Mean + SEM, n = 5 experiments, ***p<0.001, Student's t test.