β-PIX protein expression.

<p>Western blot analysis of β-PIX expression in CaCo-2, HT29 and SW480 cells, treated for 24 hours with different concentrations of rosiglitazone (1, 10, 50 µM), AS601245 (0.1, 1, 10) and combined treatment with 50 µM rosiglitazone and 0.1 µM AS601245. Equal protein loading was confirmed by exposure of the membranes to the anti-β-actin antibody. Quantification of protein products (on the right) was performed by densitometric scanning. Data are normalized using the β-actin signal and are indicated as means ± SD from three independent experiments. (A) Western blot analysis of CaCo-2 cells and relative densitometric values. (B) Western blot analysis of HT29 cells and relative densitometric values. (C) Western blot analysis of SW480 cells and relative densitometric values. Variance analysis: * p<0.05, **<i>p</i><0.01 vs control or rosiglitazone treated cells.